Operation Mousetrap is a technology entrepreneurship program designed to increase the business acumen and entrepreneurship skills of inventors and innovators and to support university technology commercialization in a rural setting. Each intensive, twelve-week program is limited to eight participants from the university community and uses various activities to strengthen the participants' business knowledge. Over three years, it has evolved into a successful program that integrates faculty and student innovation with entrepreneurship education and has resulted in start-ups, venture capital investment, and job creation. In addition, students in the College of Business are integrated into the program by providing market research, entrepreneur assessments, speaker and program evaluations, and business coaching. The program is replicable and can be implemented in both rural and urban settings with an emphasis on lessons learned from four cohorts of participants.